In atomic biology, DNA ligase is a specific blazon of enzyme, a ligase,that aliment single-stranded discontinuities in bifold abandoned DNA molecules, in simple words strands that accept double-strand breach (a breach in both commutual strands of DNA). Purified DNA ligase is acclimated in gene cloning to accompany DNA molecules together. The alternative, a single-strand break, is anchored by a altered blazon of DNA ligase application the commutual fiber as a template,but still requires DNA ligase to actualize the final phosphodiester band to absolutely adjustment the DNA.

DNA ligase has applications in both DNA adjustment and DNA archetype (see Mammalian ligases). In addition, DNA ligase has all-encompassing use in atomic analysis laboratories for Genetic recombination experiments.

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